Search results for "Mycorrhiza-resistant mutant"

Changes in polypeptide profiles of two pea genotypes inoculated with the arbuscular mycorrhizal fungus Glomus mosseae

1994

The symbiotic interaction between pea roots ('Pisum sativum' L.) and the endomycorrhizal fungus 'Glomus mosseae' should lead to specific gene expression of both symbionts. In order to detect symbiosis-related proteins (endomycorrhizins), we used two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) after phenolic extraction of total soluble proteins. Furthermore, to better characterize the molecular changes after fungal infection, two pea genotypes available in our laboratory were used: cv. Frisson (myc⁺) and an isogenic mycorrhiza-resistant mutant (myc⁻). The latter prevents intraradical fungal development. Several differences were observed in polypeptide patterns of silver-stained …